POLI
Protein-coding gene in the species Homo sapiens
DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene . [4] It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from Pol η . Pol ι, is a Y family polymerase that is involved in translesion synthesis . It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses Hoogsteen base pairing , during DNA synthesis, it will add adenine opposite to thymine in the syn conformation and can add both cytosine and thymine in the anti conformation across guanine , which it flips to the syn conformation.
References
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000038425 - Ensembl , May 2017
- ↑ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
- ↑ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
- ↑ Frank EG, Woodgate R (Aug 2007). "Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese" . J Biol Chem . 282 (34): 24689–96. doi : 10.1074/jbc.M702159200 . PMID 17609217 .
Further reading
- McIntyre J (2020). "Polymerase iota - an odd sibling among Y family polymerases" . DNA Repair . 86 (102753): 102753. doi : 10.1016/j.dnarep.2019.102753 . PMID 31805501 .
- McDonald JP, Tissier A, Frank EG, et al. (2001). "DNA polymerase iota and related rad30-like enzymes" . Philos. Trans. R. Soc. Lond. B Biol. Sci . 356 (1405): 53–60. doi : 10.1098/rstb.2000.0748 . PMC 1087691 . PMID 11205331 .
- Tissier A, Frank EG, McDonald JP, et al. (2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function". Biochem. Soc. Trans . 29 (Pt 2): 183–7. doi : 10.1042/BST0290183 . PMID 11356150 .
- McDonald JP, Rapić-Otrin V, Epstein JA, et al. (1999). "Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta" . Genomics . 60 (1): 20–30. doi : 10.1006/geno.1999.5906 . PMID 10458907 .
- Masutani C, Kusumoto R, Iwai S, Hanaoka F (2000). "Mechanisms of accurate translesion synthesis by human DNA polymerase eta" . EMBO J . 19 (12): 3100–9. doi : 10.1093/emboj/19.12.3100 . PMC 203367 . PMID 10856253 .
- Tissier A, McDonald JP, Frank EG, Woodgate R (2000). "poliota, a remarkably error-prone human DNA polymerase" . Genes Dev . 14 (13): 1642–50. doi : 10.1101/gad.14.13.1642 . PMC 316739 . PMID 10887158 .
- Tissier A, Frank EG, McDonald JP, et al. (2000). "Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota" . EMBO J . 19 (19): 5259–66. doi : 10.1093/emboj/19.19.5259 . PMC 302107 . PMID 11013228 .
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination" . Genome Res . 10 (11): 1788–95. doi : 10.1101/gr.143000 . PMC 310948 . PMID 11076863 .
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs" . Genome Res . 11 (3): 422–35. doi : 10.1101/gr.GR1547R . PMC 311072 . PMID 11230166 .
- Bebenek K, Tissier A, Frank EG, et al. (2001). "5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro" . Science . 291 (5511): 2156–9. Bibcode : 2001Sci...291.2156B . doi : 10.1126/science.1058386 . PMID 11251121 . S2CID 83716303 .
- Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing" . EMBO Rep . 1 (3): 287–92. doi : 10.1093/embo-reports/kvd058 . PMC 1083732 . PMID 11256614 .
- Frank EG, Tissier A, McDonald JP, et al. (2001). "Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template" . EMBO J . 20 (11): 2914–22. doi : 10.1093/emboj/20.11.2914 . PMC 125476 . PMID 11387224 .
- Vaisman A, Tissier A, Frank EG, et al. (2001). "Human DNA polymerase iota promiscuous mismatch extension" . J. Biol. Chem . 276 (33): 30615–22. doi : 10.1074/jbc.M102694200 . PMID 11402031 .
- Ohmori H, Friedberg EC, Fuchs RP, et al. (2001). "The Y-family of DNA polymerases" . Mol. Cell . 8 (1): 7–8. doi : 10.1016/S1097-2765(01)00278-7 . PMID 11515498 .
- Vaisman A, Woodgate R (2002). "Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines" . EMBO J . 20 (22): 6520–9. doi : 10.1093/emboj/20.22.6520 . PMC 125734 . PMID 11707422 .
- Faili A, Aoufouchi S, Flatter E, et al. (2002). "Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota". Nature . 419 (6910): 944–7. Bibcode : 2002Natur.419..944F . doi : 10.1038/nature01117 . PMID 12410315 . S2CID 4431410 .
- Kannouche P, Fernández de Henestrosa AR, Coull B, et al. (2003). "Localization of DNA polymerases eta and iota to the replication machinery is tightly co-ordinated in human cells" . EMBO J . 21 (22): 6246–56. doi : 10.1093/emboj/cdf618 . PMC 137208 . PMID 12426396 .
- Frank EG, Sayer JM, Kroth H, et al. (2002). "Translesion replication of benzo [ a ] pyrene and benzo [ c ] phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase iota" . Nucleic Acids Res . 30 (23): 5284–92. doi : 10.1093/nar/gkf643 . PMC 137958 . PMID 12466554 .
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode : 2002PNAS...9916899M . doi : 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 .
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